You can activate City of Shadows's second ability while it has no storage counters on it, though it won't add any mana in this case. You did not tap it for mana, though, in case any abilities (such as the ability of a Fertile Ground enchanting it) care about that.
04-10-2004
wotc
The storage counters aren't removed when you activate the mana ability.
Random a card of Magic: The Gathering (MTG), but you can also roll the D20 dice, random a Planeswalker, have fun with the random rules and more.